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. 2020 Jul 9;10(18):8281–8297. doi: 10.7150/thno.47315

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The expression of p68 is increased in Pkd1 mutant renal epithelial cells and tissues. (A to C) The expression of p68 in Pkd1 (WT) and Pkd1 mutant (Null) MEK cells, and in postnatal Pkd1 heterozygous PH2 (PH2) cells and Pkd1 homozygous PN24 (PN24) cells was examined by qRT-PCR (A) and Western blot (B) analysis; The relative p68 protein level was quantified from 3 independent immunoblots compared to actin (C). (D to F) The expression of p68 in postnatal day 21 kidneys from Pkd1^+/+: Pkhd1-Cre (WT) and Pkd1^fl/fl: Pkhd1-Cre (Pkd1^fl/fl) mice was examined by qRT-PCR (D) and Western blot (E, F) analysis. (G) The expression of p68 was examined in postnatal day 21 kidneys from Pkd1^+/+: Pkhd1-Cre (WT) and Pkd1^fl/fl: Pkhd1-Cre (Pkd1^fl/fl) mice with anti-p68 antibody and co-stained with LTL or DBA. (H) The expression of p68 was examined in kidneys from ADPKD patients and normal individuals with anti-p68 antibody and co-stained with LTL or DBA, which indicated that p68 expression was increased in cyst-lining epithelia in ADPKD kidneys but not in normal human kidneys. Scale bars, 50 µm.