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. 2020 Jul 1;10(18):8051–8060. doi: 10.7150/thno.43507

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Impacts of CQ on the subsets of T cells in the spleen after heart transplantation. (A) Representative images of the spleens (two pictures/group) and total numbers of splenocytes were manually calculated. (B) FCM analysis of the proportions and numbers of CD4⁺ T cells and CD8⁺ T cells from spleens harvested from each group. (C) FCM plots and histograms show the percentage of effector (CD62L^loCD44^high) CD4⁺ and CD8⁺ T cells in each group. (D) FCM plots and histograms show the ratios of Tregs (Foxp3⁺) in CD4⁺ T cells and the number of the Foxp3⁺CD4⁺ T cells. (E) FCM analysis of the percentage of CD4⁺ and CD8⁺ T cells secreting IFN-γ or IL-17A. Data are the mean ± SD and representative of three independent experiments (n=6). *p<0.05; unpaired Student's test.