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. 2020 Jul 9;10(18):8400–8414. doi: 10.7150/thno.45124

Figure 5.

Figure 5

ERO1L activity is essential for its growth-promoting role in PDAC. (A) Western blotting analysis of AsPC1 and BxPC3 cells after overexpressing either wide type ERO1L or its inactive mutant C394A. (B) Cell viability of ERO1L-WT or ERO1L-C394A overexpressing AsPC1 and BxPC3 cells (n = 3). (C) The long-term effect of ERO1L-WT or ERO1L-C394A overexpression in AsPC1 and BxPC3 cells was revealed by colony formation assay (n = 3). (D-E) AsPC1-vector, AsPC1-ERO1L-WT, and AsPC1-ERO1L-C394A cells were subcutaneously injected into the left and right hind limbs of 5 nude mice; gross xenografts (D) and tumor weights (E) were shown. *P < 0.05.