Figure 6.

ERO1L promotes the Warburg effect in pancreatic cancer cells. (A) Gene set enrichment analysis showed significant gene sets related to ERO1L expression; NES, normalized enrichment score; false discovery rate (FDR) was set at 0.25. (B) Measurement of glucose uptake in sh-Ctrl and sh-ERO1L Capan-2 and MiaPaCa-2 cells (n = 3). (C) Measurement of lactate production in sh-Ctrl and sh-ERO1L Capan-2 and MiaPaCa-2 cells (n = 3). (D) Detection of the extracellular acidification rate (ECAR) and oxygen consumption rate (OCR) in sh-Ctrl and sh-ERO1L Capan-2 and MiaPaCa-2 cells (n = 3). (E) Representative images of ERO1L expression in tumor tissues from PDAC patients who received preoperative ¹⁸F-FDG PET/CT examination; scale bar: 50 µm; the difference in the SUVmax value between ERO1L-high and ERO1L-low groups was analyzed. (F) AsPC1 and BxPC3 cells were cultured in media containing galactose but no glucose; plate colony formation experiment was performed to determine the anchorage-dependent tumor growth. (G) Effect of 2-FDG on the plate colony formation ability of ov-vector and ov-ERO1L AsPC1 and BxPC3 cells. (G) Effect of 2-DG on the plate colony formation ability of ov-vector and ov-ERO1L AsPC1 and BxPC3 cells in the presence or absence of 10 mM mannose. *P < 0.05 and **P < 0.01.