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. 2020 Jul 17;12:5945–5956. doi: 10.2147/CMAR.S245941

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© 2020 Liu et al.

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miR-186-5p was a target forMYH9. (A) MYH9 was predicted by starBase as a potential target for miR-186-5p. (B–C) Dual-luciferase reporter assay was conducted to verify the interaction between miR-186-5p and MYH9. (D) The efficiency of miR-186-5p knockdown or overexpression was shown. (E) The expression of MYH9 after the loss-and gain- experiment was measured in GC cells using Western blot. (F–G) The expression of MYH9 on GC tissues was explored at mRNA (F) and protein (G) level. (H–I) Verification of MYH9 expression pattern in GC tissues or cells. (J) A correlation analysis between MYH9 and miR-186-5p was repressed. *P<0.05.