TABLE 2.
Biodistribution assessment following subretinal injections into albino rabbit eyes (A) or subcutaneous injections into NOG mice (B)
| (A) Subretinal injections into albino rabbit eyes | |||||||
|---|---|---|---|---|---|---|---|
| Threshold cycle (Ct) (hESC‐RPE/mg; hESC/mg). Detection limit: 1 cell in 1.85 mg of rabbit tissue | |||||||
| Week 1 hESC‐RPE | Week 4 hESC‐RPE | Week 12 hESC‐RPE | Month 12 hESC‐RPE | ||||
| RPLPO (human) | Rabbit 1 | Rabbit 2 | Rabbit 3 | Rabbit 4 | Rabbit 5 | Rabbit 6 | Rabbit 7 |
| Lung | — | — | — | — | — | — | — |
| Spleen | — | — | — | — | — | — | — |
| Liver | — | — | — | — | — | — | — |
| Heart | — | — | — | — | — | — | — |
| Kidney | — | — | — | — | — | — | — |
| Optic nerve | — | 36.2 (2; 0.5) | — | — | — | — | — |
| Vitreous (eye 1) | 29.9 (213; 35) | 29.3 (331; 53) | 29.4 (300; 48) | 34.1 (8; 2) | — | 32.9 (20; 4) | — |
| Vitreous (eye 2) | 24.6 (11 282; 1245) | 29.6 (262; 43) | 30.7 (112; 20) | 34.2 (8; 2) | 34.2 (8; 2) | 34.1 (8; 2) | — |
| (B) Subcutaneous injections into NOG mice | |||||||
|---|---|---|---|---|---|---|---|
| Threshold cycle (Ct) (hESC‐RPE/mg; hESC/mg). Detection limit: 1 cell in 20 mg of mouse tissue | |||||||
| Month 7 hESC‐RPE | |||||||
| RPLPO (human) | Mouse 1 | Mouse 2 | Mouse 3 | Mouse 4 | Mouse 5 | Mouse 6 | Mouse 7 |
| Lung | — | — | — | — | — | — | — |
| Spleen | — | — | — | — | — | — | — |
| Liver | — | — | — | — | — | — | — |
| Heart | — | — | — | — | — | — | — |
| Kidney | — | — | — | — | — | — | — |
| Gonads | — | — | — | — | — | — | — |
Abbreviations: hESC, human embryonic stem cell; RPE, retinal pigment epithelial.