Figure 5. Hsp27 Upregulation Due to Neuronal Tsc1/2 Loss Is Reduced by 17-AGG and by Rapamycin in the Dose Range and within the Critical Window that Restores Ciliation.

Western blots of protein lysates and quantification at endpoint DIV13 (A-F) and DIV20 (G-L) from control and Tsc2-sh neurons treated with vehicle or with a four-day dose curve of 17-AGG. The indicated proteins are expressed as average fold changes of vehicle-treated ctrl-sh neurons. Western blot data were normalized using GAPDH as loading control. Data quantification relative to vehicle-treated Tsc2-sh neurons (n = 3–4 experiment/condition; one-way ANOVA with Dunnett’s multiple-comparison test, *p < 0.05, **p < 0.05, ***p < 0.005, ****p < 0.0001). Error bars indicate ± SEM.

(M) Western blots of protein lysates from control and Tsc2-sh neurons treated with vehicle or with 20-nM rapamycin (Rapa) for one day or four days with endpoint at DIV13.

(N and O) Quantification of Hsp27 (N, n = 3) and pS6 (O, n = 7). Western blot data normalized using GAPDH as loading control. Data are average fold changes of vehicle-treated ctrl-sh neurons ± SEM (one-way ANOVA with Dunnett’s multiple-comparison test, *p < 0.05, **p < 0.005, ****p < 0.0001).