Table 2.
Combinations of protease preparations for LPI hydrolysis in one‐step and two‐step process
| Hydrolysis ID | Number of enzymes | Endoprotease (E/S %) | ||||
|---|---|---|---|---|---|---|
| Alcalase 2.4 L | Papain | Corolase 7089 | Neutrase 0.8 L | |||
| One‐step | OS 1 | 2 | 0.5 | 0.5 | ||
| OS 2 | 3 | 0.5 | 0.5 | 0.5 | ||
| OS 3 | 3 | 0.5 | 0.5 | 1.0 | ||
| OS 4 | 2 | 0.5 | 0.5 | |||
| OS 5 | 2 | 0.5 | 0.2 | |||
| OS 6 | 2 | 0.2 | 0.5 | |||
| OS 7 | 3 | 0.5 | 0.2 | 0.5 | ||
| OS 8 | 3 | 0.5 | 0.2 | 0.5 | ||
| Two‐step | TS 1a | 2 | 0.5 (1) | 0.5 (2) | ||
| TS 2a | 2 | 0.5 (1) | 0.5 (2) | |||
| TS 3a | 2 | 0.5 (1) | 0.2 (2) | |||
| TS 4a | 2 | 0.2 (1) | 0.5 (2) | |||
Abbreviation: E/S, Enzyme to solution ratio.
a
LPI was hydrolyzed in two‐step process in the first stage for 1 hr with one enzyme (1) and after 1 hr the second enzyme (2) was added and hydrolysis was continued.