Acoustic manipulation of a 3-mm glass sphere in a pig bladder along three different paths. The sphere was levitated along the acoustic axis, moved laterally, and lowered in path 1 (A-C). In path 2 (D–F), the sphere was levitated, then moved in a circular path in a transverse plane where it moved in and out of the ultrasound imaging plane as detected by change in image intensity (Movies S5 and S6). Path 3 (G–I) was a vertical circle in yz focal plane (Movie S7). As in Fig. 3, the superimposed ultrasound images are color-coded to show the sphere’s motion; the position maps for the ultrasound are in the center; and the superimposed camera images are on the right. Each image shows the 2D projection of intended path. (Scale bar, 1 mm.)