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. 2020 Jul 6;117(29):17177–17186. doi: 10.1073/pnas.2002898117

Fig. 1.

Fig. 1.

Cyclin D1 represses HNF4α target gene expression in AML12 hepatocytes, human liver cancer cell lines, and the liver. Mouse AML12 hepatocytes and human liver cancer cell lines HepG2 and Sk-Hep1 were treated with cyclin D1 and control siRNA. (A) Western blot of cyclin D1 and HNF4α expressions in the three cell lines. (B) DNA synthesis as determined by BrdU uptake. (C) Venn diagrams of genes up-regulated and down-regulated by cyclin D1 depletion in the three cell lines. (D) Predicted activity of HNF4α as determined by IPA upstream regulatory analysis of RNA-seq following cyclin D1 knockdown in the three cells lines and following transient cyclin D1 overexpression in mouse liver. (E) GSEA of AML12 cells treated with cyclin D1 siRNA for genes that were down-regulated (Left) or up-regulated (Right) following acute HNF4α KO in mouse liver.