Table 2.
CryoEM imaging and model refinement statistics for the C. jejuni G508A flagellar filament
| Data collection | Parameter value |
| Magnification | 81,000 |
| Voltage (kV) | 300 |
| electron exposure (e-Å2) | 20 |
| Defocus range (um) | 1.25 to 2.25 |
| Pixel size (Å) | 1.08 |
| Image processing | |
| Helical rise (Å) | 4.8 |
| Helical twist (°) | 65.32 |
| Initial number of particles | 276,180 |
| Final number of particles | 116,959 |
| Map sharpening B factor (Å2) | −90 |
| Model:map FSC (0.50) | 3.5 |
| Gold-standard” map:map FSC (0.143) | 3.5 |
| Model composition (single flagellin) | |
| Nonhydrogen atoms | 4431 |
| Number of amino acid residues | 574 |
| Number of ligands | 17 |
| B factors (Å2) | |
| Protein | 76.03 |
| Ligand | 106.41 |
| Root-mean-square deviations | |
| Bond lengths (Å) | 0.006 |
| Bond angles (deg) | 0.628 |
| Validation | |
| MolProbity score | 1.89 |
| Clashscore | 7.08 |
| Rotamer outliers (%) | 0 |
| Protein geometry | |
| Ramachandran favored (%) | 91.58 |
| Ramachandran allowed (%) | 9.24 |
| Ramachandran outliers (%) | 0.17 |
| Model PDB ID | 6X80 |
| Density map EMDB ID | EMD-22088 |