Fig. 1.

Decline in reproductive performance in response to male age and mating activity. (A) Experimental design. U and F males. (B) Number of offspring from a single mating, excluding infertile matings (age and mating interaction: χ²₂ = 98.668; P = 0.0005) (n = 73–135). (C) The proportion of infertile matings (age and mating interaction: χ²₂ = 11.32; P = 0.0035) (n = 110–137). (D) Female latency to remate (age and mating interaction: χ²₂ = 34.886; P < 0.0001) (n = 60–70). (E) Paternity share of the experimental first male (age and mating interaction: χ²₂ = 219.34; P = 0.0234) (n = 37–55). (F) Number of eggs laid by virgin females or females mated to spermless (tudor) or control (cont) experimental males (U: female mating treatment: χ²₄ = 2398.7; P < 0.0001; n = 29–56) (F: female mating treatment: χ²₄ = 1405.9; P < 0.0001; n = 29–56) (G) Female latency to mate for virgin females and females first mated to spermless (tudor) or control (cont) experimental males (U: female mating treatment: χ²₄ = 137.923; P < 0.0001; n = 25–45) (F: female mating treatment: χ²₄ = 99.421; P < 0.0001; n = 23–42). Differences at P < 0.05 within mating groups and age categories and between treatments are represented as different letters.