Fig. 5.

Manipulation of the insulin signaling pathway ameliorates ejaculate deterioration in Old-F males. (A) The proportion of infertile matings (U: age: χ²₁ = 10.413; P = 0.001; line: χ²₁ = 0.437; P = 0.508; age and line interaction: χ²₁ = 1.941; P = 0.164; n = 95–202) (F: age: χ²₁ = 115.83; P < 0.0001; line: χ²₁ = 5.433; P = 0.0198; age and line interaction: χ²₁ = 3.684; P = 0.055; n = 94–189). (B) Female remating latency (U: age: χ²₁ = 28.927; P < 0.0001; line: χ²₁ = 3.602; P = 0.058; age and line interaction: χ²₄ = 0.660; P = 0.416; n = 92–198) (F: age and line interaction: χ²₁ = 4.727; P = 0.03; n = 91–186). “C” stands for control and “A” stands for ablated lines. Results are shown as means ± SEM. Shaded areas are confidence intervals at the 0.15 level. Differences at P < 0.05 within lines and age categories are represented as different letters.