Fig. 3.

Screening of the Shuffle and ShuffleStEP libraries. (A) Percentage hit rates (number of hits/number screened × 100) for the Shuffle and ShuffleStEP libraries in the crude scFv screen. (B) A comparison of IC₅₀ values for the purified scFvs originating from the Shuffle (blue), ShuffleStEP (green), H2L2, and the single-CDR mutated libraries in the EC screen compared to parent C0020187. Each data point represents the average IC₅₀ value of an antibody candidate from at least two independent tests, and the data points for parental C0020187 shows the average of measurements of different batches across seven independent experiments. A two-tailed Mann–Whitney U test was used to calculate statistical significance: ****P < 0.0001.