Fig. 4.
Binding affinities of affinity-matured antibodies to human ARG2. (A) A graphic comparison of estimated KD values of antibodies derived from the Shuffle/ShuffleStEP and pool-maturation strategies. Representative leads from the panel, which are further discussed here, are annotated in blue and pink for the Shuffle/ShuffleStEP stream and pool-maturation stream respectively. (B) Kinetic parameters of the representative leads derived on the Octet software. ka: association rate constant; kd: dissociation rate constant; KD: equilibrium dissociation constant.