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. 2020 Jul 7;117(29):16928–16937. doi: 10.1073/pnas.1820132117

Fig. 3.

Fig. 3.

Expression of viral genes in MEAM1 whiteflies during long-term retention. (A–C) Relative expression levels of V1 (A), C1 (B), and C3 (C) of TYLCV and PaLCuCNV (PaL) in whitefly whole bodies after 0 and 18 d of retention as detected by qRT-PCR. Mean ± SEM of three independent experiments is shown. P < 0.05 (one-way ANOVA, LSD test). (D–F) Relative expression levels of TYLCV V1 (D), C1 (E), and C3 (F) genes in MGs and PSGs of whiteflies after 0 and 18 d of retention as detected by qRT-PCR. Mean ± SEM of three independent experiments is shown. *P < 0.05; **P < 0.01 (independent-sample t test). (G) Localization of TYLCV Rep in MGs and PSGs of whiteflies after 0 and 18 d of retention. Rep was detected by use of a rabbit anti-Rep polyclonal antibody and goat anti-rabbit IgG labeled with Dylight 488 (green) secondary antibody. Cell nucleus was stained with DAPI (blue). The white arrow indicates the immune-reactive signal of TYLCV Rep. For each time point, 20 samples were analyzed, and a similar trend was observed.