KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER | ||
|---|---|---|---|---|
| Antibodies | ||||
| Anti-Myc | Santa Cruz Biotechnology | sc-40; RRID:AB_627268 | ||
| Anti-Flag (M2) | Sigma-Aldrich | F3165–5MG; RRID:AB_259529 | ||
| Anti-HA | Cell Signaling Technology | 3724S; RRID:AB_1549585 | ||
| Anti-hemagglutinin (HA) | Covance | MMS-101P; RRID:AB_439687 | ||
| Anti-LATS1 | Cell Signaling Technology | 3477S; RRID:AB_2133513 | ||
| Anti-phospho-LATS1 (T1079) | Cell Signaling Technology | 8654S; RRID:AB_10971635 | ||
| Anti-phospho-YAP (S127) | Cell Signaling Technology | 4911S; RRID:AB_2218913 | ||
| Anti-STRN4 | Bethyl laboratories | A304–573A-T; RRID:AB_2782004 | ||
| Anti-YAP | Wang et al., 2011 | N/A | ||
| Anti-YAP | Santa Cruz Biotechnology | sc-101199; RRID:AB_1131430 | ||
| Anti-YAP | Cell Signaling Technology | 14074S; RRID:AB_2650491 | ||
| Anti-α-tubulin | Sigma-Aldrich | T6199–200UL; RRID:AB_477583 | ||
| Anti-β-actin | Sigma-Aldrich | A5441–100UL; RRID:AB_476744 | ||
| Bacterial and Virus Strains | ||||
| DH5α competent E. coli | Laboratory of Junjie Chen | N/A | ||
| BL21 competent E. coli | Laboratory of Junjie Chen | N/A | ||
| DB3.1 competent E. coli | Laboratory of Junjie Chen | N/A | ||
| Biological Samples | ||||
| Endometrial cancer tissue arrays | US Biomax, Inc | T091 and EMC1502 | ||
| Chemicals, Peptides, and Recombinant Proteins | ||||
| Hexadimethrine bromide (Polybrene) | Sigma Aldrich | Cat# H9268 | ||
| 4’,6-diamidino-2-phenylindole (DAPI) | Sigma Aldrich | Cat# D9542 | ||
| TRIzol | Thermo Fisher | Cat# 15596026 | ||
| Power SYBR Green PCR master mix | Thermo Fisher | Cat# 4367659 | ||
| Streptavidin-conjugated beads | GE Healthcare | Cat# 17-5113-01 | ||
| Biotin | Sigma Aldrich | Cat# B4639 | ||
| S protein beads | Novagen | Cat# 69704 | ||
| Growth factor-reduced BD Matrigel matrix | BD Biosciences | Cat# 354230 | ||
| Polyethylenimine | VWR | Cat# 87001–912 | ||
| Critical Commercial Assays | ||||
| Gateway BP Clonase Enzyme Mix for BP assay | Thermo Fisher | Cat# 11789021 | ||
| Gateway LR Clonase Enzyme Mix for LR assay | Thermo Fisher | Cat# 11791043 | ||
| Script Reverse Transcription Supermix Kit for reverse transcription assay | Bio Rad | Cat# 1708841 | ||
| Deposited Data | ||||
| Proteomic data | ProteomeXchange Consortium database | Project identifier: PXD016931 | ||
| Experimental Models: Cell Lines | ||||
| HEK293T | ATCC, MD Anderson Cancer Center (Junjie Chen Lab) | N/A | ||
| MCF10A | ATCC, MD Anderson Cancer Center (Junjie Chen Lab) | N/A | ||
| HEK293A | Thermo Fisher and MD Anderson Cancer Center (Jae-Il Park Lab) | N/A | ||
| MDA11, KLE, AN3CA, MFE296, MFE319, RL95–2 | University of New South Wales (Kyle Hoehn Lab), Moffitt Cancer Center (Doug Marchion Lab) and University of Virginia (Hui Li Lab) | N/A | ||
| HEC1A, HEC1B | MD Anderson Cancer Center (Characterized Cell Line Core Facility) | N/A | ||
| Oligonucleotides | ||||
| STRN4 guide RNAs, see Table S5 | This paper | N/A | ||
| qPCR primers, see Table S5 | This paper | N/A | ||
| Recombinant DNA | ||||
| Lenti Guide-Puro vector | Sanjana et al., 2014 | Addgene #52963 | ||
| Lenti Cas9-Blast construct | Sanjana et al., 2014 | Addgene #52962 | ||
| pLKO-YAP shRNA | Zhao et al., 2008 | Addgene #27369 | ||
| Lenti-MAP4K1-SFB | This paper | N/A | ||
| Lenti-MAP4K2-SFB | This paper | N/A | ||
| Lenti-MAP4K3-SFB | This paper | N/A | ||
| Lenti-MAP4K4-SFB | This paper | N/A | ||
| Lenti-MAP4K5-SFB | This paper | N/A | ||
| Lenti-MAP4K6-SFB | This paper | N/A | ||
| Lenti-MAP4K7-SFB | This paper | N/A | ||
| SFB-STRN4-Full length (1–753 amino acids) | This paper | N/A | ||
| SFB-STRN4-ΔNT (136–753 amino acids) | This paper | N/A | ||
| SFB-STRN4-ΔCC (1–68, 137–753 amino acids) | This paper | N/A | ||
| SFB-STRN4-ΔMD (1–136, 436–753 amino acids) | This paper | N/A | ||
| SFB-STRN4-DWD (1–435 amino acids) | This paper | N/A | ||
| MBP-LATS1-C3 | Han et al., 2018 | N/A | ||
| Software and Algorithms | ||||
| Optimized CRISPR Design | CHOPCHOP website | http://chopchop.cbu.uib.no/ | ||
| ImageJ | ImageJ Software | RRID: SCR_003070 | ||
| Ingenuity Pathway software | Ingenuity Systems | https://www.ingenuity.com/ | ||