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. Author manuscript; available in PMC: 2020 Jul 25.
Published in final edited form as: Nat Protoc. 2019 Feb;14(2):313–330. doi: 10.1038/s41596-018-0102-x

Fig. 4 ∣. Isotope labelling of de novo pyrimidine biosynthesis.

Fig. 4 ∣

13C and 15N flux into nucleotides and precursors of the pyrimidine synthesis pathway from glutamine and aspartic acid in TSC2−/− cells are under mTORC1 control via CAD protein phosphorylation. In the graphs at left, white bars represent TSC2+/+ cells and black bars represent TSC2−/− cells. UMPS, uridine monophosphate synthetase. Parts of this figure reprinted with permission from Ben-Sahra et. al. 17, American Association for the Advancement of Science.