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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Ticks Tick Borne Dis. 2020 Jan 3;11(3):101369. doi: 10.1016/j.ttbdis.2019.101369

Fig 5. Immunity elicited by a cocktail of recombinant salivary proteins including Salp14 and TSLPI elicits erythema at tick bite sites.

Fig 5.

A. Sera from each of 2 guinea pigs immunized with a cocktail of 20 μg each of recombinant salivary proteins (Anti-Salp cocktail 1) or Ovalbumin (Anti-OVA) were assessed by ELISA for specific antibodies to each of the salivary proteins. About 30 clean I. scapularis nymphs were allowed to engorge on each of 2 Hartley female guinea pigs immunized with Salp cocktail 1 or OVA and the following parameters assessed: B. Visualization of redness at the tick bite sites 24 h post-tick attachment; D. Erythema over the course of feeding; E. Rate of tick detachment; F. Percent recovery of repleted ticks; and G. Engorgement weights of individual nymphs. Error bars represent means ± SEM. Significance of differences assessed in: C, and D by 2-way ANOVA with Sidak’s multiple comparison test; E and F by Mann-Whitney test. (*p< 0.05; **p<0.005).