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. 2020 Jul 24;11:3713. doi: 10.1038/s41467-020-17443-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Visualizing CMG-driven unwinding of individual Atto647N-labeled surface-immobilized fork substrates with TIRF microscopy. Unwinding of 28-bp duplex region by CMG leads to dissociation of the fluorescent strand from the surface. Representative fields of view are shown at three time points on Fork^ssLag a and Fork^dsLag b following ATP addition (n = 3 independent experiments). c Percentage of molecules unwound as a function of time for Fork^ssLag (gray, N = 1215 molecules from n = 3 independent experiments) and Fork^dsLag (blue, N = 1611 molecules analyzed). Data represent mean ± SD from three independent experiments for each substrate. Source data are provided as a Source Data file.