Fig. 5. Binding of a protein to the excluded strand promotes DNA unwinding by CMG.
a Single-turnover time-course unwinding of ForkssLag by CMG in the absence (black) and presence (orange) of RPA. b Single-turnover time-course unwinding of ForkdsLag by CMG in the absence (blue) and presence (orange) of RPA. a, b RPA was added after CMG binding to the fork. A competitor oligonucleotide was included with ATP addition to prevent further RPA and CMG binding during unwinding. Data represent mean ± SD (n = 3). c CMG-catalyzed single-turnover unwinding of fork DNA containing a single biotin on the lagging-strand arm in the absence (black) and presence (green) of streptavidin (SA). Data represent mean ± SD (n = 3 independent experiments). Solid lines are fits to Eq. (2) (Methods). Source data are provided as a Source Data file.