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. 2020 Jul 24;11:3713. doi: 10.1038/s41467-020-17443-7

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© The Author(s) 2020

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Fig. 6 — a Single-turnover unwinding of fork DNA with a single-stranded lagging-strand arm and 28 bp parental duplex. CMG was bound to the fork in the presence of ATPγS. Subsequently ATP was added and unwinding was monitored through Cy5 fluorescence. An oligonucleotide complementary to the lagging-strand arm (Comp^LagTail) was added either before (blue, Comp^LagTail→ATP) or 2 minutes after (red, ATP→Comp^LagTail) ATP. b Single-turnover unwinding of fork DNA with d(GGCA)₁₀ lagging-strand arm and 60-bp parental duplex. CMG was bound to the fork in the presence of ATPγS. Subsequently ATP was added and unwinding was monitored through Cy5 fluorescence. An oligonucleotide complementary to the lagging-strand template within the 60-bp parental dsDNA (Comp^LagParent) was added either before (blue, Comp^LagParent→ATP) or 20 minutes after (red, ATP→Comp^LagParent) ATP. Source data are provided as a Source Data file.