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. 2020 Jul 24;11(7):575. doi: 10.1038/s41419-020-02778-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative images of echocardiography exhibiting changes in cardiac function in each group. Echocardiographic analysis of left ventricular end-diastolic diameter (LVIDd) b, left ventricular end-systolic diameter (LVIDs) c, ejection fraction (EF) d, and fractional shortening (FS) e in week 4 after the first cycle of PD-1 inhibitor treatment or sham operation, n = 6 per group. f Ratio of heart weight to body weight. g Ratio of lung weight to body weight; n = 6 per group. h Representative photomicrographs of iNOS. i Quantitative analysis of iNOS-positive M1 proinflammatory macrophages, n = 3 per group; Scale bar: 50 μm. Proinflammatory cytokine iNOS j, IL-1β k, IL-6 l, and TNF-α m mRNA expression levels were examined using qRT-PCR, n = 6 per group. ^*P < 0.05 versus the control group.