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. 2020 Jul 24;11(7):575. doi: 10.1038/s41419-020-02778-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Macrophages transfected with Ad-KLF4 or Ad-Ctrl and treated with a PD-1 inhibitor. Macrophages were treated with a PD-1 inhibitor. Untreated macrophages were used as a control. a The typical results of F4/80+/iNOS+ type using flow cytometry in macrophages. b Quantitative analysis of F4/80+/iNOS+ cells were analyzed using flow cytometry. c The typical results of CD38+ type using flow cytometry. d Quantitative analysis of CD38+ cells were analyzed using flow cytometry. n = 3 per group. e–g The expression levels of M1 markers iNOS e, IL-1β f, and TNF-ɑ g mRNAs were examined using qRT-PCR. n = 6 per group. ^*P < 0.05 versus the control group; ^▲P < 0.05 versus the PD-1 inhibitor + Ad-KLF4 group.