Fig. 5. Body temperatures and viral RNA in plasma and organ samples measured during the efficacy trials.

Average rectal temperatures of vRVFV-4s vaccinated and mock-vaccinated lambs, goat kids and calves (a, d, g) post homologous RVFV challenge. Monitoring of viremia in vaccinated and mock-vaccinated lambs by RT-qPCR and virus isolation (b, e, h). Samples that tested negative are depicted at the detection limit of the PCR (1.3 log₁₀ RNA copies/ml) or virus isolation (1.55 log₁₀ TCID₅₀/ml). Detection of viral RNA in liver and spleen samples (c, f, i). The detection limit of the PCR was 2.3 log₁₀ RNA copies/g. Open symbols represent animals that died or were euthanised during the study because they reached a humane endpoint. Measurements were taken from distinct samples.