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. 2020 Jul 24;5:65. doi: 10.1038/s41541-020-00212-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Neutralizing antibody responses in time as determined by VNT (a, e, i). Challenge (red arrow) of both groups was on DPV 21. Statistical analysis by unpaired T-test of the VNT responses 1 week post vaccination and at the day of challenge (b, f, j). Detection of anti-N antibodies by ELISA in weekly obtained sera (c, g, k). Titres are expressed as percentage competition ratio of the optical densities (OD) of the sample and the OD of the negative control (% S/N). All values below 40% are considered positive, between 40 and 50% are considered doubtful and above 50% are considered negative. Statistical analysis by unpaired T-test of the N-ELISA responses 1 week post vaccination and at the day of challenge (d, h, l), (ns) not significant, asterisk (*) significant with a p value ≤ 0.05. Measurements were taken from distinct samples. Error bars represent s.d.