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. 2020 Jul 24;7:249. doi: 10.1038/s41597-020-00581-4

Fig. 4.

Fig. 4

Sanger sequencing validates the constructed cultivar specific transcriptome. Multiple sequence alignment of NPR1-1 coding sequence obtained from eight E. coli colonies (NPR1-1 seq. 1–8) by the Sanger method, assembled short or long-read cv. Rywal transcripts and Phureja DM gene model (Sotub07g016890.1.1). Grey - sequence identity, black - SNPs. The alignment was prepared and visualised with Geneious Prime 2020.1.165.