Fig. 2.

Suppressive activity of peficitinib on proinflammatory cytokine production by peripheral blood mononuclear cells

Peripheral blood mononuclear cells isolated from patients with RA (A, B, E, F) or SSc (C, D, G, H) were stimulated with anti-CD3/anti-CD28 antibodies for 24 h (A–D) or recombinant human IL-2 for 72 h (E–H) in the presence of the indicated concentration of peficitinib. Proinflammatory cytokine production in the culture supernatants were assessed by bead-based immunoassays. Cell viability was assessed with CellTiter-Glo (B, D, F, H). Data represents mean or mean with S.E.M. of three to five independent experiments using different donors. pefi: peficitinib.