Fig. 4.

Peficitinib inhibition of cytokine-induced STATs phosphorylation and chemokine production in skin fibroblasts

(A) Normal human dermal fibroblasts (NHDFs) were stimulated with IL-6 or IFN-α in the presence of indicated concentrations of peficitinib, and STAT phosphorylation was assessed by flow cytometry. Data are expressed as the mean values with S.E.M. of three independent experiments using fibroblasts from different lots. (B) Chemokine productions from IL-6- or IFN-α-stimulated NHDFs were measured by bead-based immunoassays. Data are expressed as the mean values with S.E.M. of three independent experiments using fibroblasts from a single lot. ^†The data include values below the detection limit. MFI, mean fluorescence intensity; MCP-1: monocyte chemoattractant protein-1; IP-10: IFN-γ–induced protein-10; STAT: signal transducer and activator of transcription.