TABLE 1.
Glycan structures of tested HMOs1
| HMO | Glycan | Structure |
|---|---|---|
| LNnDFH | Lacto-N-neodifucohexaose | Galβ1-4(Fucα1-3)GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc |
| LNDFH II | Lacto-N-difucohexaose II | Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc |
| DFLNH | Difucosyllacto-N-hexaose | Galβ1-4(Fucα1-3)GlcNAcβ1-6[Galβ1-3(Fucα1-4)GlcNAcβ1-3]Galβ1-4Glc |
| LNDFH I | Lacto-N-difucohexaose I | Fucα1-2Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4Glc |
| 3-FL | 3-Fucosyllactose | Galβ1-4(Fucα1-3)Glc |
| A-Type-6 | Blood Group A tetrasaccharide | GlcNAcα1-3(Fucα1-2)Galβ1-4Glc |
| LNFP II | Lacto-N-fucopentaose II | Fucα1-4(Galβ1-3)GlcNAcβ1-3Galβ1-4Glc |
| LDFT | Lactodifucotetraose | Fucα1-2Galβ1-4(Fucα1-3)Glc |
| 2′-FL | 2′-Fucosyllactose | Fucα1-2Galβ1-4Glc |
| LNT2 | Lacto-N-triose | GlcNAcβ1-3Galβ1-4Glc |
| LNFP I | Lacto-N-fucopentaose I | Fucα1-2Galβ1-3GlcNAcβ1-3Galβ1-4Glc |
| LNnT | Lacto-N-neotetraose | Galβ1-4GlcNAcβ1-3Galβ1-4Glc |
| LNnFP | Lacto-N-neofucopentaose | Galβ1-4GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc |
| LNFP V | Lacto-N-fucopentaose V | Galβ1-3GlcNAcβ1-3Galβ1-4(Fucα1-3)Glc |
| p-LNnH* | p-Lacto-N-neohexaose | Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc |
| LNnH* | Lacto-N-neohexaose | Galβ1-4GlcNAcβ1-6(Galβ1-4GlcNAcβ1-3)Galβ1-4Glc |
| LNT | Lacto-N-tetraose | Galβ1-3GlcNAcβ1-3Galβ1-4Glc |
| LNnO | Lacto-N-neooctaose | Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4GlcNAcβ1-3Galβ1-4Glc |
| LSTc | LS-tetrasaccharide c | NeuAcα2-6Galβ1-4GlcNAcβ1-3Galβ1-4Glc |
| 6′-SL | 6′-Sialyllactose | NeuAcα2-6Galβ1-4Glc |
| 3′-SL | 3′-Sialyllactose | NeuAcα2-3Galβ1-4Glc |
| LSTa | LS-tetrasaccharide a | NeuAcα2-3Galβ1-3GlcNAcβ1-3Galβ1-4Glc |
| LSTb | LS-tetrasaccharide b | NeuAcα2-6(Galβ1-3)GlcNAcβ1-3Galβ1-4Glc |
| DSLNT | Disialyllacto-N-tetraose | NeuAcα2-6(NeuAcα2-3Galβ1-3)GlcNAcβ1-3Galβ1-4Glc |
| DS-L | Disialyl lactose | NeuAcα2-8NeuAcα2-3Galβ1-4Glc |
1Fuc, fucose; Gal, galactose; Glc, glucose; GlcNAc, N-acetyl-glucose; HMO, human milk oligosaccharide; LNnH, lacto-N-neohexaose; NeuAc, N-acetyl-neuraminic acid; p-LNnH, p-Lacto-N-neohexaose.
*
p-LNnH and LNnH were not separated well in our high-performance anion-exchange chromatography system. Thus, their concentrations are given as the sum of p-LNnH + LNnH.