Table 2.
Comparison of sperm parameters (± SD) between the experimental groups after frozen-thawed and treatment with 10 μg/ml Calligonum (CGM) extract and LIPUS (pulsed mode and continues wave)
| GroupsSperm parameters | Control | Frozen -thawed | Frozenthawed+CGMextract | Frozenthawed+continueswave of ultrasound | Frozenthawed+continueswave oftrasound+CGMextract | Frozenthawed+pulsedmode ofultrasound | Frozenthawed+pulsedmode of ultrasound +CGMextract |
|---|---|---|---|---|---|---|---|
| Viability (%) | 94.17c ± 5.97 | 87.52 ± 4.75 | 90.11 ± 6.12 | 82.76b ± 5.68 | 86.70 ± 7.12 | 81.82b ± 2.87 | 87.94 ± 4.25 |
| Total motility(%) | 89.37 ± 3.74 | 81.30 ± 3.74 | 85.42 ± 4.28 | 76.76ab ± 5.29 | 81.65 ± 5.18 | 75.86ab ± 3.47 | 81.90 ± 3.93 |
| Progressivemotility (%) | 38.80 ± 2.57 | 34.81a ± 3.28 | 37.81 ± 4.13 | 33.04ab ± 2.98 | 37.24 ± 5.11 | 32.60ab ± 3.58 | 36.18a ± 4.78 |
| Normalmorphology (%) | 30.11 ± 3.16 | 26.58 ± 3.54 | 28.41 ± 4.27 | 27.58 ± 3.95 | 30.82 ± 2.75 | 28.35 ± 4.82 | 30.05 ± 3.66 |
a; Significant difference with control group in the same row (P≤0.05), b; Significant difference with freeze and thawed group + CGM extract, in the same row P≤0.026, and c; Significant difference with other groups in the same row (P≤0.047).