. 2020 Jul 15;14(2):91–101. doi: 10.22074/ijfs.2020.6065

Table 2.

Comparison of sperm parameters (± SD) between the experimental groups after frozen-thawed and treatment with 10 μg/ml Calligonum (CGM) extract and LIPUS (pulsed mode and continues wave)


Parameters	Control	Low dose	Medium dose	High dose

TBW (g)	36.12 ± 2.82^a	36.31 ± 3.06^a	36.38 ± 3.67^a	37.31 ± 3.01^a
TW (g)	0.12 ± 0.011^a	0.12 ± 0.009^a	0.12 ± 0.008^a	0.10 ± 0.010^b
BWA (g)	4.62 ± 0.67^a	5.24 ± 1.56^ab	5.58 ± 2.53^ab	6.97 ± 1.15^b
Testosterone (ng/ml)	6.88 ± 0.32^a	6.44 ± 0.30^a	6.22 ± 0.53^a	5.10 ± 0.57^b
STsD (μm)	194.38 ± 4.33^a	187.48 ± 5.56^a	173.32 ± 5.78^b	161.96 ± 5.45^c
GEH (μm)	58.97 ± 3.48^a	57.36 ± 2.36^a	50.02 ± 1.79^b	43.69 ± 3.28^c
TCT (μm)	13.47 ± 1.27^a	14.07 ± 2.09^a	16.41 ± 1.93^a	20.70 ± 2.58^b
LCs (No/mm²)	37.35 ± 2.79^a	36.82 ± 2.11^a	33.57 ± 2.30^a	28.72 ± 2.97^b
SCs (No/one tubule)	22.76 ± 1.37^a	22.79 ± 1.64^a	20.72 ± 1.83^a	16.68 ± 1.55^b
Johnsen’s score	9.42 ± 0.26^a	9.35 ± 0.30^a	8.64 ± 0.39^b	7.52 ± 0.47^c
Sperm count (×10⁶)	34.66 ± 1.65^a	31.55 ± 1.42^b	27.44 ± 1.81^c	19.22 ± 1.48^b
Sperm motility (%)	85.06 ± 2.32^a	81.95 ± 3.32^a	74.34 ± 1.25^b	62.40 ± 2.98^c
Sperm viability (%)	89.22 ± 1.56^a	86.66 ± 2.73^a	79.33 ± 1.80^b	72.55 ± 2.12^c
DNA damage sperms (%)	5.11 ± 1.36^a	7.55 ± 1.94^a	11.22 ± 2.16^b	19.33 ± 2.12^c
Abnormal sperms (%)	10.33 ± 0.86^a	11.66 ± 1.22^a	15.44 ± 1.87^b	19.88 ± 1.69^c

All data are presented as mean ± SD. Low dose; 40 mg/kg aspartame-treated, Medium dose; 80 mg/kg aspartame-treated, High dose; 160 mg/kg aspartame-treated. TBW; Total body weight, TW; Testicular weight, BWA; Body weight alternations, STsD; Seminiferous tubules diameter, GEH; Germinal epithelium height, TCT; Testicular capsule thickness, LCs; Leydig cells, and SCs; Sertoli cells. Different superscripts in the same row show significant differences between groups (P<0.05).