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. 2020 Jul 17;2020:2892917. doi: 10.1155/2020/2892917

Figure 5.

Figure 5

miR-21-5p targets TIMP3 in HCC cell lines. (a) The predicted miR-21-5p binding sites in the 3′-UTR of the TIMP3 gene. (b) The relative Luciferase activities were determined using a Dual-Luciferase Reporter Assay Kit following cotransfection with TIMP3-wt or TIMP3-mut and miR-21-5p mimic or NC mimic in HepG2 and HCCLM3 cells. (c) TIMP3 mRNA expression in HepG2 and HCCLM3 cells was measured by RT-qPCR assay following transfection with miR-21-5p inhibitor or NC inhibitor in HepG2 and HCCLM3 cells. (d) Western blotting was applied to quantify the expression level of TIMP3 following transfection with miR-21-5p inhibitor or NC inhibitor in HepG2 and HCCLM3 cells. The experiment was repeated three times; results were shown as means ± standard deviation. ∗P < 0.05 and ∗∗P < 0.01 vs. NC inhibitor group; ##P < 0.01 vs. NC mimic group.