Skip to main content
. 2020 Jul 17;2020:2892917. doi: 10.1155/2020/2892917

Figure 6.

Figure 6

Effects of miR-21 inhibition and/or TIMP3 silencing on cell proliferation in HCC cell lines exposed to curcumin. (a, b) HepG2 and HCCLM3 cells were transfected with miR-21 inhibitor or si-TIMP3 sequence for 24 h. RT-qPCR was applied to quantify the expression of miR-21 and TIMP3. &P < 0.05 and &&P < 0.01. (c, d) HepG2 and HCCLM3 cells were transfected with miR-21 inhibitor and/or si-TIMP3 sequence for 6 h and then treated with 40 μM curcumin incubated for additional 24 h. Cell viability was evaluated by CCK-8 assay. The experiment was repeated three times; results were shown as means ± standard deviation. ∗∗P < 0.01 vs. control group; %P < 0.05 and %%P < 0.01 vs. curcumin group; and #P < 0.05 and ##P < 0.01 vs. miR-21 inhibitor+ si-TIMP3+ Cur group.