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. 2020 Jul 17;2020:3296192. doi: 10.1155/2020/3296192

Figure 4.

Figure 4

Effect of TRAF6 on differentiation and stemness markers in gastric cancer cells. (a) Real-time PCR analysis for the differentiated gastric epithelial marker CK18 and the cancer stem-like cell marker Lgr5 in NC and TRAF6 siRNA-transfected HGC-27 cells (n = 3, ∗∗p < .005; n = 3, ∗∗∗p < .00001). (b) Real-time PCR assay for the expression of CK18 and Lgr5 in NC and TRAF6 siRNA-transfected MGC-803 cells (n = 3, ∗∗∗p < .001; n = 3, ∗∗∗p < .001). (c) Real-time PCR assay for the expression of Lgr5 and CK18 in vector and TRAF6 plasmid-transfected HGC-27 cells (n = 3, ∗∗∗p < .00001; n = 3, ∗∗∗p < .00001).