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. 2020 Jul 16;2020:3426902. doi: 10.1155/2020/3426902

Figure 1.

Figure 1

DHCR24 overexpression protects MIN6 cells from cell death caused by TM exposure. (a) Immunocytochemical (IC) analysis against c-myc (green fluorescent signal) was used to determine the transduction efficiency of Ad-DHCR24-myc. Scale bar, 100 μm. (b) Sterol contents in MIN6 cells were measured 48 h after infection with the indicated adenoviruses (50 PFU/cell). Mean ± S.D. (n = 3). (c) Images of MIN6 cells were acquired with a phase-contrast microscope 24 h after TM exposure (0, 0.5, and 1 μg/ml). Scale bar, 100 μm. (d) Numbers of adherent cells are expressed as percentage of cells in the Ad-LacZ group at 0 μg/ml TM exposure. Mean ± S.D. (n = 3); ∗p < 0.05 vs. Ad-LacZ.