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. 2020 Jul 16;2020:9768713. doi: 10.1155/2020/9768713

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Copyright © 2020 Yan Huang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of EVs derived from N2A cells on apoptosis of UC-MSCs upon OGD/R24H insult. UC-MSCs were precultured with two various concentration EVs, normal cultured medium, and R24H-N2A-CMs for 24 hours, respectively. Following treatment, UC-MSCs were subjected to OGD/R24H insult. (a–b) Apoptosis of UC-MSCs as evaluated by flow cytometry with Annexin-V/PI staining assay. (c) Apoptosis of UC-MSCs as evaluated by LDH leakage assay. (d) Cell viability of UC-MSCs as evaluated by MTT assay. (e) Expression and quantitative data of cleaved-caspase3, cleaved-caspase9, and cytochrome C as evaluated by western blotting. (f–g) Apoptosis of UC-MSCs as evaluated by TUNEL immunofluorescence. Scale bar = 50 μm. EVs-1: represented as higher concentration EVs recorded by NTA assay. EVs-2: represented as lower concentration EVs recorded by NTA assay. All data are presented as the mean value ± SD (n = 3). ∗p < 0.05; ∗∗p < 0.01, compared with normal culture medium group.