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. 2020 Jul 16;2020:9768713. doi: 10.1155/2020/9768713

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Copyright © 2020 Yan Huang et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hypoxia preconditioned enhances the survival of UC-MSCs upon OGD/R insult. UC-MSCs were cultured under hypoxia or normoxia preconditioning before non-OGD/R and OGD/R insult. (a–c) The expression of HIF-1α in normoxia and hypoxia preconditioned UC-MSCs group as evaluated western blotting and mRNA. (d) Expression and quantitative data of bax and bcl-2 were determined by western blotting. (e–f) Apoptosis of UC-MSCs as assessed by flow cytometry assay. (g) Apoptosis of UC-MSCs as determined by LDH leakage assay. (h) Cell viability of UC-MSCs as examined by MTT assay. (i) Levels of ROS as determined by dihydroethidium-DCFH-DA assay. (j) Levels of ATP as determined by firefly luciferase assay. (k) Levels of SOD as examined by WST-8 assay. (l) Levels of T-AOC as measured by ABTS assay. All data are presented as the mean value ± SD (n = 3). ∗p < 0.05; ∗∗p < 0.01, hypoxia group compared with normoxia group.