Fig. 3.

The amount of identified key mutations increase through the bottom-up convergence of mutated features on a multiscaled annotation framework. a The convergence of mutated genes and regulatory features onto pntAB and sthA operons from a Δpgi ALE experiment on E. coli K-12 MG1655 [21, 22]. b Proportions of mutated features according to different genomic feature types. The distributions were generated by finding the proportion of each mutated feature per ALE experiment. c The proportions of mutated feature types across ALE experiments that were involved in significant convergence (P < 0.05, permutation test, mutations to feature > 1) on the multiscale annotation framework. The number of times each feature type was observed mutated is included (‘n’). (d) Proportion of mutated features across ALE experiments that had significant convergence up to an annotation type on the multiscale annotation framework of Fig. 1