Table 2.
The sequence of primer used for RT-PCR in this study
| Gene | Accession No. | 5′-3′ | Tm (°C) |
|---|---|---|---|
| RUNX2 | NM_001015051 | F: TAGATAGTGATTGCGTTTGGCTATG | 60 |
| R: CACTAAGAAATGTTTCAAGGGTCC | 60 | ||
| ALP | NM_003064 | F: GAAAGTCCTTCAAAGCTGGAGTCT | 60 |
| R: TCTGGCACTCAGGTTTCTTGTATC | 60 | ||
| SPARC | NM_001309443 | F: TGTGATCTAAATCCACTCCTTCCA | 60 |
| R: ACAAACCATCCAAACATTTTAAACA | 60 | ||
| Wnt2 | NM_004185 | F: GGGGCACGAGTGATCTGTG | 62 |
| R: GCATGATGTCTGGGTAACGCT | 62 | ||
| Wnt7b | NM_058238 | F: CACAGAAACTTTCGCAAGTGG | 60 |
| R: GTACTGGCACTCGTTGATGC | 60 | ||
| β-catenin | XM_024453360 | F: TTGAACTGTTTGAGGCGAAGAG | 60 |
| R: ACTGAACACCGAGTTAGAGGAAT | 60 | ||
| GAPDH | NM_001256799.2 | F: GAACGGGAAGCTCACTGG | 60 |
| R: GCCTGCTTCACCACCTTCT | 60 |