Fig. 6.

MAPK4 knockout enhances the sensitivity of cervical cancer cells to radiation treatment and PARP1 inhibitors. a 4 weeks post-implantation, image of corresponding xenograft tumors derived from subcutaneous implantation of SiHa or caSki MAPK4 WT cells with no radiation treatment (MAPK4 WT + 0Gy), MAPK4 WT cells with radiation treatment (MAPK4 WT + 5Gy), MAPK4 KO cells with no radiation treatment (MAPK4 KO + 0Gy), MAPK4 KO cells with radiation treatment (MAPK4 KO + 5Gy). Volumes of the xenograft tumors were measured weekly and shown as line diagrams. b Relative protein levels of p-AKT, AKT, p-DNA-PK, MAPK4 and γH2AXin corresponding xenograft tumors derived from subcutaneous implantation of SiHa or caSki MAPK4 WT cells with no radiation treatment (MAPK4 WT + 0Gy), MAPK4 WT cells with radiation treatment (MAPK4 WT + 5Gy), MAPK4 KO cells with no radiation treatment (MAPK4 KO + 0Gy), MAPK4 KO cells with radiation treatment (MAPK4 KO + 5Gy), as determined by western blotting. c 4 weeks post-implantation, image of corresponding xenograft tumors derived from subcutaneous implantation of SiHa or caSki MAPK4 WT cells (MAPK4 WT), MAPK4 WT cells with olaparib treatment (MAPK4 WT + Olaparib), MAPK4 KO cells (MAPK4 KO), MAPK4 KO cells with olaparib treatment (MAPK4 KO + Olaparib). Volumes of the xenograft tumors were measured weekly and shown as line diagrams. Data are expressed as the mean ± SEM