Fig. 7.

Exosomal miR-124-3p regulates MYH9 by directly targeting the 3′-UTR. a, b Luciferase reporter assay confirmed that MYH9 was the target gene of miR-124-3p. c Immunoprecipitation of the Ago2/RISC (RNA-induced silencing complex) using the Pan-Ago2 antibody in microglia overexpressing miR-NC or miR-124-3p. IgG was used as a negative control and GAPDH was used as an internal control. d The mRNA expression level of MYH9 in microglia after miR-124-3p knockdown and overexpression by qRT-PCR. e Western blot analysis of MYH9 in microglia after miR-124-3p overexpression and knockdown. *P < 0.05