Fig. 1 |. Expansion of plasmablasts and depletion of multiple innate immune cell subsets in the periphery of patients with COVID-19.

a, Demographics, sample characteristics and disease course of patients with COVID-19. b, UMAP dimensionality reduction embedding of peripheral blood mononuclear cells (PBMCs) from all profiled samples (n = 44,721 cells) colored by donor of origin. IDs of patients with COVID-19 (n = 7) begin with ‘C’ and are colored in shades of orange (patients who were not ventilated at the time of draw) or red (patients with ARDS who were ventilated at the time of draw) and those of healthy donors begin with ‘H’ (n = 6) and are colored in blues. c, UMAP embedding of the entire dataset colored by orthogonally generated clusters labeled by manual cell type annotation. d, Proportions of each cell type in each sample colored by donor of origin. The x axes correspond to the ventilation or ARDS status of each patient. Shown are exact two-sided P values by the Wilcoxon rank-sum test. n = 6, n = 4 and n = 4 biologically independent samples for Healthy, NonVent and ARDS, respectively. Boxplot features: minimum whisker, 25th percentile − 1.5 × inter-quartile range (IQR) or the lowest value within; minimum box, 25th percentile; center, median; maximum box, 75th percentile; maximum whisker, 75th percentile + 1.5 × IQR or greatest value within.