Table 2. Comparison of substitution in Nicotiana species.

Types	Nicotiana knightiana	Nicotiana rustica	Nicotiana paniculata	Nicotiana obtusifolia	Nicotiana glauca
A/G	222	219	245	244	110
C/T	226	223	237	250	128
A/C	105	104	117	153	97
C/G	40	39	50	52	34
G/T	130	129	135	148	87
A/T	63	61	77	102	53
Total	786	775	861	847	509
Location wise distribution
LSC	560	559	630	671	327
SSC	183	184	198	210	100
IR	43	32	33	68	82

Notes.

Nicotiana tabacum was used as reference for SNPs detection.