Table 4. Mutational hotspots among Nicotiana species.

S. No	Region	Nucleotide Diversity	T. No’s of mutation	Region Length
1	infA	0.2594	45	249
2	rps12 intron	0.1527	161	527
3	rps16-trnQ-UUG	0.0845	225	1,266
4	trnK-UUU-rps16	0.0483	46	703
5	trnH-psbA	0.0438	19	433
6	rpl36-infA	0.0294	3	116
7	ccsA-ndhD	0.0287	17	237
8	rps16-intron	0.0278	27	862
9	rpl32-trnL-UAG	0.0261	61	931
10	trnM-CAU-atpE	0.0224	24	218
11	rpl33-rps18	0.0222	20	180
12	petD-rpoA	0.0198	9	182
13	rpl14-rpl16	0.0184	10	119
14	ndhE-ndhG	0.0173	7	219
15	rps15-ycf1	0.0171	17	385
16	ndhH-rps15	0.0166	4	108
17	petG-trnW-CCA	0.0157	4	127
18	petL-petG	0.0153	6	182
19	trnG-GCC-trnfM	0.0152	11	228
20	rpoA-rps11	0.0151	2	66