Table 3.

An overview of isothermal amplification methods commonly used in combination with CRISPR sensing.

Name	Description	Typical time	Typical temperature	Refs
NASBA	Nucleic acid sequence-based amplification is a method used to amplify RNA.	~30 min	41 °C	Pardee et al. (2016)
LAMP	Loop-mediated isothermal amplification is a single tube technique for the amplification of DNA. It uses 4–6 primers, which form loop structures to facilitate subsequent rounds of amplification.	15–60 min	~65 °C	(Li et al., 2019; Mukama et al., 2020a; Qian et al., 2020)
RCA	Rolling circle amplification starts from a circular DNA template and a short DNA or RNA primer to form a long single stranded molecule.	~120 min	20–37 °C	Wang et al., 2020
RPA	Recombinase polymerase amplification is a low temperature DNA and RNA amplification technique.	5–60 min	37–42 °C	(Y. Chang et al., 2019; English et al., 2019; Kellner et al., 2019; Khan et al., 2019; Sullivan et al., 2019; X. Wang et al., 2020; Williams et al., 2019)
SDA	Strand Displacement Amplification (SDA) employs a restriction endonuclease, which is capable of nicking of its recognition site, and a DNA exonuclease deficient polymerase, which is capable of initiating synthesis at a nick.	90 min	37–55 °C	Zhou et al., 2018