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Transiently transfected cells (A) are compared with the newly developed stable cell system (B), each expressing the A3AR-LgBiT and SmBiT-miniGαi constructs. The left panel shows the corrected activation profiles obtained by testing a concentration range (evaluated in duplicate) of the A3AR agonist 2-Cl-IB-MECA, the right panel shows the corresponding sigmoidal concentration–response curve. Data of one representative experiment are shown.
