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Experimental groups of chickens in immunization and challenge experiment
| Groups | Strains | Immunization (dose) | Challenge(dose) |
|---|---|---|---|
| I | / | Normal saline/0.2 mL | / |
| II | / | Normal saline/0.2 mL | 5.0 × 104/0.5 mL |
| III | pValac/pSIP409 | 1.0 × 109 CFU/0.2 mL | 5.0 × 104/0.5 mL |
| IV | pValac-EtMIC2/pSIP409 | 1.0 × 109 CFU/0.2 mL | 5.0 × 104/0.5 mL |
| V | pValac-EtMIC2/pSIP409-FnBPA | 1.0 × 109 CFU/0.2 mL | 5.0 × 104/0.5 mL |
| VI | pValac-EtMIC2-IL18/pSIP409-FnBPA | 1.0 × 109 CFU/0.2 mL | 5.0 × 104/0.5 mL |
