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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: J Cell Physiol. 2020 Jan 27;235(10):6767–6778. doi: 10.1002/jcp.29571

Figure 3. Arginylation of BiP during treatment with ART, ERA, or BOR.

Figure 3.

(A) HCT116 cells were treated with ART (50 μM) or BOR (50 nM) in the presence/absence of MG132 (10 μM) for 24 h. Western blotting analysis of BiP and arginylated BiP (R-BiP) was performed after treatment with indicated agents. Actin was used as a loading control. Densitometry analysis of the bands from the R-BiP or BiP was performed (right panel). The values are indicated as mean ± SD from three independent experiments. p-values: *, 0.05; **, 0.01; ***, 0.001. (B) HCT116 cells were treated with ERA (50 μM) in the presence/absence of MG132 (10 μM) for 24 h. Whole-cell extracts were analyzed with immunoblotting assay using indicated antibodies. Actin was used as a loading control. Densitometry analysis of the bands from the R-BiP or BiP was performed (right panel). The values are indicated as mean ± SD from three independent experiments. p-values: ***, 0.001.