Fig. 3.

Viperin inhibits the proliferation of PEDV in IPEC-J2 cells. (a) Verification of intracellular viperin protein expression with beta-actin as an internal reference. (b) Virus proliferation measured by TCID₅₀ after transfection of IPEC-J2 cells with a viperin overexpression plasmid and infection with PEDV 24 h later. (c) Expression of viperin, detected by RT-qPCR after transfection of IPEC-J2 cells with an siRNA targeting viperin. (d) Virus proliferation measured by TCID₅₀ assay in IPEC-J2 cells infected with PEDV 24 h after transfection with an siRNA targeting viperin